AddEventListener Assigned More Than Once

If in a situation, the addEventListener is being assigned more than once for same action, what will happen then? Suppose on frame 5 there is sample_btn.addEventListener(MouseEvent.CLICK… and when user navigates the application/site it comes on frame 5 again and again. Will the addEventListener be applied more than once on that button? If yes then what will happen on clicking on that button? will the associated function be called more than once now?