Image quality takes a dive when I move everything from frame 1 to frame 3

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I’m not sure what’s going on here. I decided I need to add a preloader to my site so I moved everything in frame 1 of the main stage to frame 3 so I could add in the preloader. Without the preloader, I tested it to make sure everything was moved over to frame 3 and that everything was working and now when I preview the movie in Internet Explorer all of the images (png format) are really jagged around the edges as if AA is not on anymore. Everything looks fine if its in frame 1, yet in frame 3 it looks bad. Any idea what’s up and how to correct this?