The best lossless fullscreen presentation option?

Okay I just did a 2minute spot for our trade show booth at work (came out pretty tight) in After Effects. Now what I need to do is make these projector files so the can be used as teasers to be playing on monitors around the booth. The monitor res will be around 1024-768. Now I have rendered them as raw quicktime (animation) from AE. Drug them through squeeze. Now initially I used .flv and the file size went from 2gb to 16-30mb. but the drop wasnt lossless. From squeeze they will go to flash and to projector. Now my issue is in squeeze. I am not sure what setting to use to get the best lossless video. File size is not a issue since it will be played natively. I tried importing into flash without compression but flash automatically does its own. I need the best looking projector output I can get. Any ideas?